In vitro maturation of human iPS-derived neuroepithelial cells influences transplant survival in the stroke-injured rat brain.
Tissue Eng Part A. 2017 Jun 08;:
Authors: Payne SL, Anandakumaran PN, Varga BV, Morshead CM, Nagy A, Shoichet MS
Stem cell transplantation is a promising strategy for brain tissue regeneration; yet, despite some success, cell survival following transplantation remains low. Here we demonstrate that cell viability is enhanced by control over maturation of neuronal precursor cells, which are delivered in an injectable blend of hyaluronan (HA) and methylcellulose (MC) (HAMC). We selected three subpopulations of human neuronal precursor cells derived from a cortically-specified neuroepithelial stem cell (cNESC) population based on differences in expression of multipotent and neuron-specific proteins: early, mid-, and late-differentiated neurons. These cells were transplanted into an endothelin-1 stroke-injured rat brain and their survival and fate were investigated one week later. Significantly more cells were found in the brain after transplanting early or mid- differentiated cNESCs compared to the late-differentiated population. The mid-differentiated population also had significantly more β-III tubulin-positive cells than either the early or late-differentiated populations. These results suggest that maturity has a significant impact on cell survival following transplantation, and that cells with an intermediate maturity differentiate to neurons.
PMID: 28594288 [PubMed - as supplied by publisher]