Donnelly Centre for Cellular and Biomolecular Research

What We Offer

We offer sequencing, library preparation, and other services. Take a look at what’s available at the DSC, and then proceed to Getting Started to take the first step in your project.

Sequencing

We accept libraries prepared by you and submitted directly to us for sequencing. Use the table below to explore which instrument best fits your project – grey cells indicate available run modes and Illumina’s expected data output in gigabases.

Parameters and output for the Donnelly Sequencing Centre's Illumina MiSeq, NextSeq, and NovaSeq sequencers.

Want to try the NovaSeq but need less data than a full flowcell? Not a problem: we’ll match your libraries with other compatible projects to fill up a flowcell – this can be a great way to stay within your budget. Please note, however, that this can increase turnaround time as we wait for other projects – filling up a flowcell with your own samples is your best bet for getting data back quickly. The DSC prides itself in having highly competitive turnaround times - our typical turnaround times for delivery of data following sample submission are less than 12 business days depending in the sequencing application and volume.

Library preparation

We also offer complete library preparation services for a wide variety of sample types and analysis needs.

RNA

DNA

mRNA-Seq – stranded
NEB NEBNext Ultra II Directional RNA
NEB NEBNext DSC Long-Read Protocol
Illumina TruSeq Stranded mRNA
Illumina TruSeq DSC Long-Read Protocol

mRNA-Seq – non-stranded
Takara SMART-Seq v4 Ultra-Low*

3’ RNA-Seq
Illumina SureCell WTA 3’
Takara SMART-Seq v4 3’ DE*

Ribo-depletion RNA-Seq – stranded
NEB NEBNext Ultra II Directional RNA
Takara SMARTer Stranded RNA-Seq
Takara SMARTer Universal Low Input RNA
Illumina TruSeq Stranded Total Ribo-Zero

Small RNA-Seq
NEB NEBNext Small RNA
Takara SMARTer smRNA-Seq

Targeted RNA-Seq
Takara SMARTer Target RNA Capture*
Illumina TruSeq RNA Exome (stranded)


 


 

*with Illumina Nextera library prep

Shotgun sequencing
NEB NEBNext Ultra II DNA
Illumina Nextera DNA Flex
Illumina Nextera DNA XT
NEB NEBNext FFPE**

Amplicon sequencing and 16S rRNA
Illumina amplicon PCR
Illumina 16S rRNA metagenomics

Mate-pair sequencing
Illumina Nextera Mate Pair

Whole-exome sequencing
Illumina TruSeq DNA Exome
Illumina Nextera DNA Exome
Illumina Nextera Flex for Enrichment
Agilent SureSelect XT2 Exome

ChIP-Seq
NEBNext Ultra II DNA for ChIP
Illumina TruSeq ChIP-Seq
Takara DNA SMART ChIP-Seq

Methylation
NEB EpiMark Methylated DNA**
Illumina TruSeq Methyl Capture EPIC
Illumina TruSeq DNA Methylation
Takara EpiXplore Meth-Seq

CRISPR Screen
Custom methods (single and dual guides)

**with NEB NEBNext Ultra II DNA

Please feel free to contact us to help select the best protocol for your application. If you’re looking for something not listed here, or for a novel protocol altogether, the DSC team is also happy to collaborate on method development.

Data distribution and storage

Sequence data are distributed on our servers as de-multiplexed compressed FASTQ files. Our default multiplex settings are trimming enabled, with 1 index mismatch allowed.

For users who are newer to NGS and bioinformatics, we recommend trying Illumina’s BaseSpace application. If you’ve signed up for an account, include your BaseSpace information on your Sample Information Sheet and we’ll upload your data directly there (timing dependent on network availability). This will allow you to make use of their many cloud-based sequencing analysis applications, including BWA, Cufflinks, TopHat, Velvet, Issac, RNAexpress, DNAstar, and FASTQC. Sign up and explore their toolset at Illumina.

Raw data (and samples) will be stored at the DSC for 3 months after project completion; demultiplexed fastq files are stored for two weeks.

Other services

Preparing libraries yourself but missing some key equipment? The DSC offers the following services:

  • DNA or RNA quantification: ThermoFisher Qubit (low-throughput) or Quant-iT (high-throughput), broad range or high sensitivity kits

  • RNA fragment analysis: Agilent TapeStation HS RNA ScreenTape or Bioanalyzer RNA Pico Chip

  • Library distribution analysis: Agilent TapeStation HS D1000 ScreenTape or BioAnalyzer HS DNA Chip

  • Library quantification: NEBNext Library Quant qPCR

Contact us about making use of other basic pieces of lab equipment (e.g. thermocycler, heating block, tube or plate centrifuge).