What We Offer
We accept libraries prepared by you and submitted directly to us for sequencing. Use the table below to explore which instrument best fits your project – grey cells indicate available run modes and Illumina’s expected data output in gigabases.
Want to try the NovaSeq but need less data than a full flowcell? Not a problem: we’ll match your libraries with other compatible projects to fill up a flowcell – this can be a great way to stay within your budget. Please note, however, that this can increase turnaround time as we wait for other projects – filling up a flowcell with your own samples is your best bet for getting data back quickly. The DSC prides itself in having highly competitive turnaround times - our typical turnaround times for delivery of data following sample submission are less than 12 business days depending in the sequencing application and volume.
We also offer complete library preparation services for a wide variety of sample types and analysis needs.
mRNA-Seq – stranded
mRNA-Seq – non-stranded
Ribo-depletion RNA-Seq – stranded
*with Illumina Nextera library prep
Amplicon sequencing and 16S rRNA
**with NEB NEBNext Ultra II DNA
Please feel free to contact us to help select the best protocol for your application. If you’re looking for something not listed here, or for a novel protocol altogether, the DSC team is also happy to collaborate on method development.
Data distribution and storage
Sequence data are distributed on our servers as de-multiplexed compressed FASTQ files. Our default multiplex settings are trimming enabled, with 1 index mismatch allowed.
For users who are newer to NGS and bioinformatics, we recommend trying Illumina’s BaseSpace application. If you’ve signed up for an account, include your BaseSpace information on your Sample Information Sheet and we’ll upload your data directly there (timing dependent on network availability). This will allow you to make use of their many cloud-based sequencing analysis applications, including BWA, Cufflinks, TopHat, Velvet, Issac, RNAexpress, DNAstar, and FASTQC. Sign up and explore their toolset at Illumina.
Raw data (and samples) will be stored at the DSC for 3 months after project completion; demultiplexed fastq files are stored for two weeks.
Preparing libraries yourself but missing some key equipment? The DSC offers the following services:
DNA or RNA quantification: ThermoFisher Qubit (low-throughput) or Quant-iT (high-throughput), broad range or high sensitivity kits
RNA fragment analysis: Agilent TapeStation HS RNA ScreenTape or Bioanalyzer RNA Pico Chip
Library distribution analysis: Agilent TapeStation HS D1000 ScreenTape or BioAnalyzer HS DNA Chip
Library quantification: NEBNext Library Quant qPCR
Contact us about making use of other basic pieces of lab equipment (e.g. thermocycler, heating block, tube or plate centrifuge).